Lab Research Tools

360° Movie of Pediatric Retinal Research Lab (PRRL) in the ERI at Oakland University.

Services, Lab Safety Training, BioSafety Training, Animal Research Training, Human Subjects Research Training

The First Ever Map of Changes in RNA Polymerase-II Occupancy in Maturing Photoreceptor Gene Promoters in vivo, (mouse) by the Mitton Lab at Oakland University. We found a way to do it without having to dissociate neural retinas or sort cells ;).

The Mitton Lab uses this bibliography, PDF manager. Works with WORD & OfficeLibre. IIt is free and multiplatform. Get it if you want to do research with Dr. Mitton at Mendeley.com

Better Curve Fitting: R-project https://www.r-project.org/ and drc package Consult with Dr. Mitton for installation of the R environment and how to use the drc package for dose-response curve fitting. mitton@oakland.edu

Illumina DNA-Sequencing Information and Tools

Local Run Manager Information at Illumina.com
iSeq100 Software Tools
Illumina Massively Parallel DNA Sequencing Introduction Dr Eric Chow UCSF
Purifying DNA During Next Generation Sequencing Library Preparation with Magnetic Beads Dr Eric Chow UCSF
How to use the Agilent BioAnalyzer to check DNA (or RNA) Dr Eric Chow UCSF
Illumina Ampliseq Custom Panel Design

Human Genetics Tools: Useful for Pediatric Retinal Disease DNA-Sequencing

e!Ensembl Tools (USWest Server) EMBL-EBI (VEP is here)
Gene2Phenotype Database from EMBL
Medical Genetics and Human Variation Tools at NCBI (CLINVAR is here)
GnomAD Browser at Broad Institute

Useful Eye, Retina, Biochemistry Information

Retinal Ganglion Cell: single cell gene expression database from Neurodegeneration Lab UCONN Health
The Retina Reference website- by Dr. David Browning, MD PhD
Molecular Vision website – the Journal
Retinal Information Network – RetNet
Timing of Mouse Retinal Development – Kenneth Mitton, PhD FARVO
How Oxygen Regulates Gene Expression – Hypoxia Inducible Factor-1 (HIF-1) Nobel Prize 2019 in Physiology or Medicine.

Dr. Mitton’s Recommended Guides to Practical Laboratory Methods in Biochemistry: For those who desire to work or study in my lab group, these are useful introductions to some of the methods at use in our research. If you are just interested in cell culture, protein purification, gene expression analysis and DNA sequencing, then you will find these educational too.